Conference Proceeding

Selective killing of cancer cells by fruit extract of Cucumistrigonus: Components and its in vitro cytotoxicity and in vivo studies

Ms. Santilna ,
Lecturer, JSS College of Pharmacy, India.

Ms. Santilna has completed her Bachelor of Pharmacy and Masters of Pharmacy (Pharmacology) from JSS College of Pharmacy Mysore, India. Later she started working as a lecturer in Karuna College of Pharmacy, Palakkad, Kerala. Presently she is working as a lecturer in JSS College of Pharmacy, Udhagamandalam, Tamil Nadu, India.

The present study was carried out to determine the antitumor activity of Cucumistrigonus fruits extracts against cancer cell lines namely Heal, HEp-2, A-549, MCF-7, MDA-MB 231, HT-29 and C-6 and two normal (L-929, Vero) cell lines. The Cucumistrigonus fruits were extracted using n-hexane, toluene, chloroform, ethyl acetate, hydro alcoholic and aqueous solvents and it's in vitro cytotoxicity studies have been carried out. Among the extracts, toluene and chloroform extracts showed a potential activity (CTC50 < 1µg/ml).TLC profiling of toluene extract namely fraction F23 and F24 showed highest cancer cell killing activity (CTC50 <0.1 µg/ml). The active chloroform extract obtained from Cucumistrigonus was found to reduce the tumor and associated symptoms in DLA induced as cites tumor bearing mouse. This study indicates that the toluene and chloroform extracts of Cucumistrigonus possesses significant antitumor activity in vitro which may be due to high cytotoxicity toward the cancer cell line. Our studies encourage the development of novel, efficient and less toxic plant derived molecular for the cancer chemotherapy.

Published: 11 May 2017