Conference Proceeding

Positively Charged Lipid Bilayer as AFM Substrate

Ms Laila Balobaid,
Alfaisal University, Saudi Arabia

Laila Balobaid did her BSc in Physics at King Saud University. She earned her MSc in NanoBio Science at UCD in Dublin while also working on photoreduction of metallic nanoparticles on proton exchanged metal oxide crystals, and also on AFM imaging of structural confirmation of DNA molecules on surfaces at the London Centre for Nanotechnology at UCL.

Atomic force microscopy (AFM) is a surface-probing technique that can image biomolecules at subnanometre resolution under near-physiological conditions, i.e., in aqueous solution. For AFM imaging to be successful, it requires samples that are gently adsorbed on a flat substrate. Lipid membranes have been employed as substrates for AFM experiments on a range of biomolecules, including DNA and the molecular motor Myosin V. Here, dioctadecyldimethylammonium bromide (DDAB) vesicles were self-assembled on muscovite mica surface in water to form a positively charged lipid bilayer membrane suitable for adsorbing and studying negatively charged biomolecules. AFM was used to first characterise the membranes and next to visualise adsorbed DNA in aqueous solution.

Published: 27 April 2017