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Targeted Editing of Avian Herpesvirus Vaccine Vector using CRISPR/Cas9 Nuclease

Research Article


Herpesvirus of turkey (HVT) is an avian alphaherpesvirus used as live vaccine against Marek's disease for more than 40 years. Here, we describe the application of CRISPR/Cas9-based genome editing as a rapid and efficient method of introducing targeted mutations to the genome of a live herpesvirus vaccine vector using a transfection-infection system.

Published: 15 Nov 2016

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