Conference Proceeding

Evaluation of DNA Damage Induced by heavy metal in the lobster Panulirus homarus Through Comet Assay

Dr. S. L. Sasikala,
Toxicity of heavy metals is discussed in detail. We are exposed to Aluminium through various sources such as drinking water, food and pharmaceutical products – medicines, antacids, vaccines, dialysis treatments etc. Aluminium toxicity causes chronic renal failure, neurofibrillary degeneration, damage of cholinergic system. Aluminium chloride is a genotoxic agent causing DNA damage. In aquatic organisms DNA damage is associated with reduced growth and survival of embryos, larvae and adult. A study was carried out in the lobster, Panulirus homarus to assess early changes induced by aluminium exposure via direct route. Apart from brain cells and reproductive cells concurrently, aluminium bioaccumulation was studied in the somatic tissues as well to understand the potential genotoxic biomarker responses. Cell viability monitored prior to the lysis step using trypan blue showed substantial decrease in the viability of brain, ovarian, sperm and liver cells after aluminium exposure. The genotoxicity in the tissues of lobster exposed to aluminium chloride assessed through the extent of DNA damage using comet assay depicted little or no DNA damage in control while the damage was very obvious in aluminium exposed tissues. Aluminium chloride was found to damage sperm cells and egg cells of the lobster H. americanus. It is obvious that there is every possibilities for such DNA damaged sperm cells and egg cells have very little chances of embryo formation nor even fertilization resulting in affected growth, reproduction and population dynamics.

Published: 18 July 2017


Copyright: © 2017 S. L. Sasikala. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.