Conference Proceeding

Effect of Triterpene Fraction of Cucumistrigonus on Apoptosis, Cell Cycle Arrest and Cell Proliferation in Cancer Cells

Ms.Sandhya Chipurupalli,
J.S.S College of Pharmacy, Ootacamund, Tamil Nadu, India

Ms. Sandhya Chipurupalli Master of Pharmacy (M. Ph) in Pharmacology from Sri Sai Aditya Inst. Of Pharmaceutical Sciences & Research, Affiliated to Andhra University, India. Later she started working as a assistant professor in VJ’S college of Pharmacy (Andhra University), Rajahmundry, E. G. Dt, A.P, India. Presently she is working as Research Scholar, Department of Pharmacology, JSS College of Pharmacy, India.

Taxol based combination chemotherapy remains the predominant treatment for cancer. However, taxane related drug resistance and neurotoxicity have promoted us to develop substitute treatment strategies. Kinesis spindle protein which is crucial for bipolar spindle formation and duplicate chromosome separation in early phase of mitosis, emerged as attractive target for cancer chemotherapy. The aim of the study was to investigate the mechanism of kinesis spindle protein inhibition that plays an essential role in establishing mitotic spindle bipolarity, by the novel small molecular inhibitor. Anti proliferative activity of triterpene fraction of Cucumistrigonus (TF25CT) in cancer cell line was accessed by cell viability assay (MTT, SRB). TF25CT showed dose dependent inhibition of cancer cell proliferation. The anti proliferative effect of TF25CT was associated with induction of apoptosis as well as morphology changes and DNA fragment. The morphology of Apoptosis nuclei was quantified using Hoechst staining and PI fragmentation. The degree of DNA fragmentation was analyzed by agars gel electrophoresis. The observations indicate that induced apoptotic- like morphological changes such as cell shrinkage and blabbing. Suppression of microtubule dynamics is responsible for the ability of TF25CT to inhibit the mitotic progression and cell proliferation using fluorescent microscope, we examined the effect of TF25CT on dynamics of individual microtubule in two living human cancer cell line. Collectively, the kinesis protein inhibitor represents an imported compound for further investigation as novel anticancer remedial.

Published: 11 May 2017