Conference Proceeding

Can Epigenetic Modifiers Advance the Stem Cell Differentiation

Dr. Ahmed Taher El-Serafy,
College of Medicine, University of Sharjah, UAE

Epigenetic modifiers are those chemical able to affect the epigenetic signature of the genome and consequently the gene expression. Historically, an additive role for various modifiers was suggested to affect stem cell differentiation. The logic behind was rendering the genome more active and more responsive to the culturing conditions. We showed earlier that different modifiers have certain preference to activate specific lineages of differentiation. Our studies in monolayer and three dimensional pellet cultures showed that the DNA demethylating agent (5-Aza-dC) can activate the osteogenic differentiation while the histone deacetylases inhibitor, Trichostatin A, induced the chondrogenic differentiation. The combination of the two agents had no additive effects. Many authors tried, through microarray approach, to investigate the gene expression profile after the administration of these agents. The overall result was that not all genes are susceptible to the agents’ effect. Our recent data suggested that certain transcription pathways were more susceptible for each of the modifiers. Extending our studies to the adipogenic differentiation, 5-Aza-dC and the histone deacetylase inhibitor ‘Suberoylanilidehydroxamic acid’ showed opposing effect. The next generation sequencing showed activation of different pathways, even being under the same culturing condition. This presentation will include a general review on the effect of these modifiers, their role in stem cell differentiation based on our recent findings as well as their potential use as an additive component in tissue regeneration programs.

Published: 27 April 2017