Conference Proceeding

Anterograde monosynaptic transneuronal tracers derived from Herpes Simplex Virus 1 strain H129.

Zhao Fei

Neurotropic viruses may infect neural system, causes damages, and lead various diseases, but their capacity of infecting and transmitting among neurons can be utilized for in neuroscience research. Multiple neurotropic viruses have been modified and broadly applied as viral tools to trace neuronal circuit. Rabies virus (RV) and pseudorabies virus (PRV) represent retrograde mono- and multisynaptic tracers, but anterograde tracer with high transsynaptic labeling efficiency or monosynaptic transmitting capacity remains lacking.

Herpes simplex virus type 1 strain 129 (H129) is preferable to transmit among neurons anterogradely. Based on H129, our group has generated a serial of recombinant viruses for neuronal circuit tracing, including H129-G4 and H129-ΔTK-tdT. H129-G4 is a multisynapticantegrade tracer with strong labeling intensity. It is capable of transmitting through multiple synapses, labeling the neurons by green florescent protein, and visualizing the morphological details of the labeled neurons. With the Tyrosine Kinase (TK) gene deletion, H129-ΔTK-tdT neither replicates nor spreads in neurons alone. With the assistance of the helper virus complimentarily expressing TK, H129-ΔTK-tdT replicates in the initially infected neuron, transmits anterogradely through one synapse, and labeled the postsynaptic neurons with tdTomato. H129-ΔTK-tdT is also capable to map the direct projectome of the specific neuron type in the given brain regions in Cre transgenic mice. The H129-ΔTK-tdT anterograde monosynaptic tracing system offers a useful tool for mapping the direct output in neuronal circuitry.

Published: 17 October 2017


Copyright: © 2017 Zhao Fei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.